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United States Department of Agriculture

Agricultural Research Service

Research Project: EFFICIENT MANAGEMENT AND USE OF ANIMAL MANURE TO PROTECT HUMAN HEALTH AND ENVIRONMENTAL QUALITY

Location: Food Animal Environmental Systems Research Unit

Title: Spatial and Temporal Changes in the Microbial Community in an Anaerobic Swine Waste Treatment Lagoon

Authors
item Cook, Kimberly
item Rothrock, Michael
item Lovanh, Nanh
item Sorrell, John - WESTERN KY UNIVERSITY
item Loughrin, John

Submitted to: Conference on Gastrointestinal Function
Publication Type: Abstract Only
Publication Acceptance Date: February 10, 2009
Publication Date: April 20, 2009
Citation: Cook, K.L., Rothrock Jr, M.J., Lovanh, N.C., Sorrell, J., Loughrin, J.H. 2009. Spatial and Temporal Changes in the Microbial Community in an Anaerobic Swine Waste Treatment Lagoon. Conference on Gastrointestinal Function.

Technical Abstract: Swine slurry is stored in pits beneath confinement buildings or in adjacent lagoons. This slurry is a valuable resource for crop fertilization and soil conditioning, but may also be a source of unpleasant odors. Microorganisms are crucial to all of the important processes that occur in anaerobic storage systems. Therefore, understanding more about how the microbial community functions should aid in developing better strategies for management and use of stored slurries. In this study, both quantitative (real-time polymerase chain reaction) and qualitative (denaturing gradient gel electrophoresis, cloning, sequence analysis) molecular analyses were used to track spatial and temporal changes in the microbial community of swine slurry from a 0.4 ha anaerobic lagoon over a period of one year and at four depths within the lagoon. Sub-populations within the bacterial community may be responsible for many important processes in the swine lagoon. Therefore, in addition to monitoring changes in the broader lagoon population (total cells, Clostridium/Eubacterium and Bacteroides), microbial groups important to odor production were specifically targeted including sulfate-reducing bacteria (SRB), methanogens, acetogens, photosynthetic organisms, and others. The concentration and diversity of Bacteroides sp. was seasonal (up to 90% decrease between March and June). Hespellia sp. and other clostridial species, on the other hand, were endemic in the slurry (concentrations up to 1.0 X 107 cells mL-1 slurry) regardless of time of the year or lagoon depth. Similarly, some genes targeted to specific functional groups were detected throughout the year (i.e., those for urea and methane production), while others were seasonal (acetogens, phototrophs and nitrifiers). The effect of depth was only seen for phototrophs and acetogens (both absent in the bottom of the lagoon). These results suggest that there were seasonal effects on the microbial community in the swine lagoon, while the effect of depth was not as pronounced. Seasonal changes in the microbial community in stored wastes may be (directly or indirectly) correlated with changes in malodor emissions from lagoons.

Last Modified: 8/27/2014