Title: Pathogenicity of recombinant H5N1 avian influenza viruses with truncated NS1 gene in chickens Authors
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2009
Publication Date: July 11, 2009
Citation: Jadhao, S., Swayne, D.E., Lee, C., Suarez, D.L. 2009. Pathogenicity of recombinant H5N1 avian influenza viruses with truncated NS1 gene in chickens [abstract]. American Association of Avian Pathologists Annual Meeting, July 11-15, 2009, Seattle, Washington. CD-ROM, paper No. 7586. Technical Abstract: The NS1 protein of influenza A virus plays an important role in blocking the induction of type I interferon and other regulatory functions in infected cells. However, differences in length of the NS1 protein has been observed in highly pathogenic H5N1, H5N2, and H7N1 subtype avian influenza viruses. To evaluate the influence of truncated NS1 gene on H5N1 virus pathogenicity in chickens, using reverse genetics, we generated polybasic H5 hemagglutinin (HA) containing A/turkey/Ireland/83 and A/chicken/Indonesia/7/03 viruses with full length and truncated NS1 genes. The viruses were compared by intravenous pathotyping and mucosal challenge in 4 week old chickens to determine how NS1 gene truncation affects the virulence.