ARS | Publication request: Mapping a stripe rust resistance gene YrC591 in wheat variety C591 with SSR and AFLP marker

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 20, 2008
Publication Date: October 23, 2008
Citation: Li, Y., Niu, Y.C., Chen, X. 2008. Mapping a stripe rust resistance gene YrC591 in wheat variety C591 with SSR and AFLP marker. Theor. Appl. Genet. 118:339-346.

Interpretive Summary: Stripe rust is one of the most destructive diseases of common wheat. To determine inheritance of stripe rust resistance and map the resistance gene(s) in wheat variety C591, progenies derived from a cross of C591 with a susceptible variety Taichung 29 were inoculated with Chinese stripe rust race CY32 in the greenhouse. Genetic analysis identified a single dominant gene, temporarily designated YrC591. Molecular markers developed using the SSR and AFLP techniques were used to map the resistance gene. SSR marker Xcfa2040-7B, and SCAR marker SC-P35M48 derived from AFLP marker P35M48373 were identified to be closely linked to the resistance gene with genetic distances of 8.0 and 11.7 cM, respectively. The SSR markers mapped the resistance gene on wheat chromosome arm 7BL. In the seedling test with five stripe rust races, the reaction patterns of C591 were different from wheat cultivars or lines carrying Yr2 or Yr6 that also are found on chromosome 7B. The results indicate that YrC591 is probably a novel stripe rust resistance gene.

Technical Abstract: Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici (PST), is one of the most destructive diseases of common wheat (Triticum aestivum L.). To determine inheritance of stripe rust resistance and map the resistance gene(s) in wheat variety C591, F1, F2, and F3 progenies derived from the Taichung 29 X C591 cross were inoculated with Chinese PST race CY32 in the greenhouse. Genetic analysis identified a single dominant gene, temporarily designated YrC591. A total of 178 SSR and 130 AFLP markers were used to test the parents and resistant and susceptible bulks. From the bulk segregant analysis, seven polymorphic SSR and two AFLP markers were selected for genotyping the F2 population. SSR marker Xcfa2040-7B, and SCAR marker SC-P35M48 derived from AFLP marker P35M48373 were identified to be closely linked to the resistance gene with genetic distances of 8.0 and 11.7 cM, respectively. The SSR markers mapped the resistance gene on chromosome arm 7BL. In the seedling test with five PST races, the reaction patterns of C591 were different from wheat cultivars or lines carrying Yr2 or Yr6 that also are found on chromosome 7B. The results indicate that YrC591 is probably a novel stripe rust resistance gene.