|Qin, Bolin - INTEGRITY/NUTRACEUTICALS,|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 20, 2009
Publication Date: April 10, 2009
Citation: Qin, B., Polansky, M.M., Dawson, H.D., Anderson, R.A. 2009. Cinnamon Extract Improves TNF-a Induced Overproduction of Intestinal ApolipoproteinB-48 Lipoproteins. Meeting Abstract. Technical Abstract: TNF-alpha stimulates the overproduction of intestinal apolipoproteins. We evaluated whether a water extract of cinnamon (Cinnulin PF®) improved the dyslipidemia induced by TNF-alpha in Triton WR-1339 treated hamsters, and whether Cinnulin PF® inhibits the TNF-alpha-induced over the secretion of apoB-48 in hamster enterocytes. In vivo, acute oral intake of Cinnulin PF® (50 mg per kg BW), inhibited the postprandial overproduction of apoB-48 lipoproteins and serum triglycerides. Ex vivo radio-labeling studies, demonstrated that CE (10 and 20 µg/mL) inhibited the over secretion of apoB-48 induced by TNF-alpha treated enterocytes. To determine the effects on the mRNA levels for the related genes of lipoprotein metabolism in enterocytes, fresh isolated primary enterocytes of hamsters were pre-treated with TNF-alpha(10 ng per mL for 30 min), then treated with and without Cinnulin PF® (10ug/mL) for 0.5, 2h, and 4h. Quantitative real-time PCR assays demonstrated that Cinnulin PF® improved the mRNA expression of IR, IRS1, IRS2, PI3K, and Akt1 and decreased the mRNA expression of MTP, PTEN, IL-alpha, Il-beta, and IL6 and TNF-alpha in TNF-alpha treated enterocytes. In summary, our results suggest that a water extract of cinnamon reverses TNF-alpha-induced overproduction of intestinal apoB-48 via regulating the gene expression of components of the insulin signaling and lipoprotein and inflammatory pathways. (Funded by USDA/ARS and Integrity Nutraceuticals).