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United States Department of Agriculture

Agricultural Research Service

Research Project: AQUATIC ANIMAL DIAGNOSTICS, PATHOGENESIS AND APPLIED EPIDEMIOLOGY

Location: Aquatic Animal Health Research

Title: An immobilization antigen gene of the fish-parasitic protozoan Ichthyophthirius multifiliis strain ARS-6

Authors
item Xu, Dehai
item Panangala, Victor
item Van Santen, Vicky - AUBURN UNIVERSITY
item Dybvig, Kevin - AUBURN UNIVERSITY
item Abernathy, Jason - AUBURN UNIVERSITY
item Klesius, Phillip
item Russo, Riccardo
item Liu, Zhanjiang - AUBURN UNIVERSITY

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: September 14, 2008
Publication Date: September 22, 2008
Citation: Xu, D., Panangala, V.S., Van Santen, V.L., Dybvig, K., Abernathy, J.W., Klesius, P.H., Russo, R., Liu, Z. 2008. An immobilization antigen gene of the fish-parasitic protozoan Ichthyophthirius multifiliis strain ARS-6. Genbank. Accession Nos. FJ012354, FJ19440.

Technical Abstract: Ichthyophthirius multifiliis (Ich) is a severe fish parasite that causes ‘white spot’ disease in many freshwater fish and leads to high mortality. The antigens on the parasite surface are involved in the antibody-mediated immobilization and hence designated as immobilization antigens (i-antigens). The nucleotide sequence of an i-antigen gene of Ichthyophthirius multifiliis strain ARS-6 was deduced. The i-antigen coding sequences and flanking sequences were determined from three overlapping PCR products. The amino terminal sequences of purified i-antigen peptide of strain ARS-6 was deduced by mass spectrometry and a partial sequence comprised of a 1.1 kb (central region) coding sequence was determined by PCR amplification of ARS-6 genomic DNA using primers based on previous i-antigen gene sequence in the GenBank. The 5’ portion of the coding sequence and 269 nucleotides of upstream sequence were determined from a PCR product generated by inverse PCR using primers based on the sequence determined from the central portion. The 3’ portion of the coding sequence plus 49 nucleotides of downstream sequences through the polyadenylation site were determined from an amplified and cloned cDNA fragment generated by a rapid amplification of cDNA ends (RACE) procedure. Two overlapping nucleotide sequences of 1627-bp and 1383-bp were obtained, trimmed and aligned to obtain a complete i-antigen gene sequence of ARS-6.

Last Modified: 7/25/2014
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