ARS | Publication request: Fine Mapping of Hessian Fly Resistance Gene H26 in an Aegilops tauschii Population

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 27, 2008
Publication Date: January 10, 2009
Citation: Yu, G., Harris, M.O., Cai, X., Xu, S.S. 2009. Fine Mapping of Hessian Fly Resistance Gene H26 in an Aegilops tauschii Population. Plant and Animal Genome Conference. Jan 10-14, 2009 San Diego Ca

Technical Abstract: Hessian fly [Mayetiola destructor (Say)] is one of the most important insect pests in wheat (Triticum L.) around the world. Among the 32 genes known to confer wheat resistance to Hessian fly, the H26 is important because it is effective against several highly virulent Hessian fly populations. The H26 was derived from Aegilops tauschii, D-genome donor species of common wheat, and was previously mapped to the wheat chromosomal deletion bin 3DL3-0.81-1.00 in a synthetic hexaploid wheat population. The objective of this study was to proceed with fine mapping of H26, using a set of newly-developed PCR-based markers. We developed a mapping population of approximately 3,000 F2 individuals derived from the cross between the resistant Ae. tauschii accession CIae 25 and the susceptible accession AL8/78. A subpopulation of approximately 90 F2 individuals was used to identify molecular marker loci closely linked to H26. So far, 10 PCR-based markers have been mapped to a small region that harbors the H26 gene. Among these 10 markers are six newly-developed STS markers that all were mapped within 2 cM from the H26 gene. These newly developed STS markers will further facilitate the high resolution mapping towards the cloning of the H26 gene.