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United States Department of Agriculture

Agricultural Research Service

Research Project: ENHANCING GENETIC MERIT OF DAIRY CATTLE THROUGH GENOME SELECTION AND ANALYSIS Title: Profiling miRNA Expression in Bovine Tissues by Deep Sequencing

Authors
item Matukumalli, Lakshmi - GEORGE MASON
item Alexander, Leeson
item McDaneld, Tara
item Smith, Timothy
item Schroeder, Steven
item Sonstegard, Tad
item Van Tassell, Curtis

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 5, 2009
Publication Date: January 12, 2009
Citation: Matukumalli, L.K., Alexander, L.J., Mcdaneld, T.G., Smith, T.P., Schroeder, S.G., Sonstegard, T.S., Van Tassell, C.P. 2009. Profiling miRNA Expression in Bovine Tissues by Deep Sequencing. Plant and Animal Genome Conference.

Technical Abstract: miRNA are short RNA sequences ( ~ 21 nt long) that have been recently identified and were found to play an important role in gene regulation and controlling major cellular processes. Several miRNA are found to be evolutionarily conserved among the mammalian species. Some miRNAs are even conserved between primates and C.elegans. We have performed deep sequencing of miRNA on Illumina genome analyzer from two pooled samples to capture the overall diversity and to identify several novel and unique miRNAs specific to Bovine species. We also performed deep sequencing on six individual cattle tissue samples (fetal testes piRNA, adult testes piRNA, fetal testes miRNA, adult testes miRNA, fetal liver miRNA and adult liver miRNA) to characterize the differential expression between tissues and across various developmental stages. We have also performed gene expression profiling on the same cattle tissues and hence their mutual expression patterns were analyzed to understand the interaction of the mRNA-miRNA with respect to their mode of action. We are currently sequencing additional 40 tissue samples and latest results from all these analyses will be presented.

Last Modified: 8/30/2014