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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED PLANT GENETIC RESOURCES FOR PASTURES AND RANGELANDS IN THE TEMPERATE SEMIARID REGIONS OF THE WESTERN U.S. Title: Distinguishing Glyceria Species of Western North America

Authors
item Bushman, Shaun
item Sedegui, Mohammed - OREGON DEPARTMENT OF AG
item Osterbauer, Nancy - OREGON DEPARTMENT OF AG

Submitted to: Seed Technology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 19, 2009
Publication Date: June 1, 2009
Citation: Bushman, B.S., Sedegui, M., Osterbauer, N. 2009. Distinguishing Glyceria Species of Western North America. Seed Technology Journal 31:66-76.

Interpretive Summary: There are seven North American and three introduced European Glyceria species growing in western North America, yet distinguishing among the species is challenging. As contaminants in annual ryegrass grass seed lots, the introduced species G. declinata and G. fluitans are undesirable domestically, while North American species are undesirable in seed grown for international trade. In order to distinguish between the western North American and introduced European Glyceria species we designed PCR, Taqman SNP, and DNA sequencing assays. The PCR assays are co-dominant markers in which a larger sized amplification product is detected in G. declinata, G. fluitans, and G. fluitans-like G. occidentalis than is detected among the other species. The Taqman SNP assay shows VIC hybridization signal for G. declinata and FAM hybridization signal for G. fluitans and G. fluitans-like G. occidentalis. DNA sequencing of the chloroplast trnK region, and the nuclear ribosomal ITS-1 region, provide several SNPs that identify each of the individual species. However, Glyceria occidentalis samples contain chloroplast and ITS-1 sequences identical to either G. leptostachya or G. fluitans, thus currently cannot be distinguished with DNA markers.

Technical Abstract: There are seven North American and three introduced European Glyceria species growing in western North America, yet distinguihsing among the species is challenging. As contaminants in annual ryegrass grass seed lots, the introduced species G. declinata and G. fluitans are undesirable domestically, while North American species are undesirable in seed grown for international trade. In order to distinguish between the western North American and introduced European Glyceria species we designed PCR, Taqman SNP, and DNA sequencing assays. The PCR assays are co-dominant markers in which a larger sized amplification product is detected in G. declinata, G. fluitans, and G. fluitans-like G. occidentalis than is detected among the other species. The Taqman SNP assay shows VIC hybridization signal for G. declinata and FAM hybridization signal for G. fluitans and G. fluitans-like G. occidentalis. DNA sequencing of the chloroplast trnK region, and the nuclear ribosomal ITS-1 region, provide several SNPs that identify each of the individual species. However, Glyceria occidentalis samples contain chloroplast and ITS-1 sequences identical to either G. leptostachya or G. fluitans, thus currently cannot be distinguished with DNA markers.

Last Modified: 11/24/2014
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