ARS | Publication request: Antagonists' impact on enzymatic response in wilt infected cotton plants

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: June 10, 2008
Publication Date: July 26, 2008
Citation: Gulyamova, T.G., Ruzieva, D.M., Nasmetova, S.M., Karimova, K.M., Khodjibaeva, S.M., Stipanovic, R.D. 2008. Antagonists' impact on enzymatic response in wilt infected cotton plants [abstract]. American Phytopathological Society. p. 610.

Technical Abstract: A number of PR-proteins possess enzymatic activity. As such, these proteins maybe indicators of defensive response of plants. Thus, we have conducted a comparative analysis of beta-1,3-glucanase, peroxidase and xylanase activity in cotton plants to determine how these enzymes are affected by the pathogen Verticillium dahliae and selected antagonists (i.e., Stachybotrys chartarum 295, Bacillus sp. 234, Streptomyces roseoflavus 33, Streptomyces sp. 7 and Trichoderma viride 445). Analysis of enzymatic activities in the cytosol and intercellular liquid obtained from cotton plants of resistant variety Bukhara-6 revealed that enzymatic activity of intercellular liquid of leaves of control healthy plants was higher than in the cell free extracts in the majority of pathogen treated plants. After infection, there was a three fold increase of glucanase and peroxidase activity, whereas xylanase activity increased by almost 10 fold. Glucanase activity was reduced in infected plants treated with antagonist and increased in uninfected plants. In contrast, peroxidase activity increased in infected plants treated with antagonist, and peroxidase activity increased in uninfected plants treated with the antagonists S. chartarum 295 and Streptomyces sp. 7. Xylanase activity increases both in healthy and infected plants on treatment with all antagonists except with antagonists Bacillus sp. 234 and T. viride 445. In general, the data reveals a positive impact of antagonists on appearance of enzymatic response in infected plants.