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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL AND PROTECTION TOOLS FOR INTEGRATED PEST MANAGEMENT OF MOSQUITOES AND FILTH FLIES

Location: Mosquito and Fly Research Unit

Title: Identification of genes specifically expressed during heat shock treatment in Aedes aegypti

Authors
item Zhao, Liming
item Wei Pridgeon, Yuping
item Becnel, James
item Clark, Gary
item Linthicum, Kenneth

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 17, 2008
Publication Date: N/A

Technical Abstract: Temperature is important for mosquito development and physiological response. Several genes of heat shock protein (HSP) families are known to be expressed in mosquitoes and may be crucial in responding to stress induced by elevated temperature. Suppression subtractive hybridization (SSH) was used to identify target transcripts to heat shock treatment in adult Aedes aegypti. SSH is a method in which two populations of mRNA are compared to obtain clones of genes expressed in one population but not in the other. Subtraction was performed in both directions enriching for cDNAs differentially expressed between non heat shock control and heat shock treatment. Heat shock treatment of adult Ae. aegypti was carried out for 1 hr at 42 ºC. Clones from differentially expressed genes were evaluated by sequencing. Target transcripts up-regulated by heat shock included 5 different HSP gene families as well as 27 other genes, such as cytochrome c oxidase, serine-type enodpeptidase, and glutamyl aminopeptidase. Additionally, some novel genes, cytoskeleton and ribosomal genes were found to be differentially expressed, and three novel up-regulated sequences belonging to a low-abundance class of transcripts were obtained. Up-regulated/down-regulated transcripts from heat shock treatment were further confirmed and quantified by quantitative real-time PCR. High temperatures can alter the gene expression of a vector mosquito population, and further characterization of these differentially expressed genes will provide information useful in understanding the genetic response to heat shock treatment, which can then be used to develop novel approaches to genetic control.

Last Modified: 8/22/2014