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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND BIOCHEMICAL MECHANISMS OF RESISTANCE TO BARLEY AND CEREAL YELLOW DWARF VIRUSES AND FUNGI

Location: Crop Production and Pest Control Research

Title: Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors

Authors
item SCOFIELD, STEVEN
item BRANDT, AMANDA

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: April 10, 2010
Publication Date: June 11, 2012
Citation: Scofield, S.R., Brandt, A.S. 2012. Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors. Methods in Molecular Biology. 894:93-112.

Interpretive Summary: Virus-induced gene silencing (VIGS) is a useful functional genomics tool for rapidly creating gene knockout phenotypes that can be used to infer gene function. Until recently, VIGS has only been possible in dicotyledonous plants. However, the development of vectors based on barley stripe mosaic virus (BSMV) has now made VIGS possible in barley and wheat. VIGS has particular advantages for functional genomics in wheat, where the organism’s hexaploidy and recalcitrance to transformation have greatly hindered strategies for functional identification of genes. Here methods are presented for using the Barley stripe mosaic virus VIGS system (BSMV-VIGS) to silence genes in hexaploid wheat.

Technical Abstract: Virus-induced gene silencing (VIGS) is a useful functional genomics tool for rapidly creating gene knockout phenotypes that can be used to infer gene function. Until recently, VIGS has only been possible in dicotyledonous plants. However, the development of vectors based on barley stripe mosaic virus (BSMV) has now made VIGS possible in barley and wheat. VIGS has particular advantages for functional genomics in wheat, where the organism’s hexaploidy and recalcitrance to transformation have greatly hindered strategies for functional identification of genes. Here methods are presented for using the Barley stripe mosaic virus VIGS system (BSMV-VIGS) to silence genes in hexaploid wheat.

Last Modified: 9/29/2014
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