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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF GENETIC RESOURCES FOR VITIS, PRUNUS, JUGLANS, FICUS, OLEA, PISTACIA, PUNICA, DIOSPYROS, ACTINIDIA, AND MORUS

Location: National Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes

Title: Isozyme variation in lychee (Litchi chinensis Sonn.)

Authors
item Aradhya, Mallikarjuna
item Zee, Francis
item Manshardt, R. - UNIV OF HAWAII - HORT

Submitted to: Scientia Horticulturae
Publication Type: Popular Publication
Publication Acceptance Date: February 2, 1995
Publication Date: November 1, 1995
Citation: Aradhya, M.K., Zee, F.T., Manshardt, R.M. 1995. Isozyme variation in lychee (Litchi chinensis Sonn.). Scientia Horticultureae 63: 21-35.

Interpretive Summary: A genetic diversity analysis involving 49 lychee (Litchi chinensis SOM.) accessions using eight enzyme systems encoding 12 loci (Zdh-I, Zdh-2, Mdh-2, Per-l, Pgi-2, Pgm-1, Pgm-2, Sk& Tpi-1, Tpi-2, Ugpp-1, and Ugpp-2) revealed moderate to high levels of genetic variability. Cluster analysis of the isozyme data from 40 genetically different accessions of the total 49 identified three groups at the 50% level of genetic similarity, the largest of which contained 32 of the 40 accessions distributed in three sub-groups. The groups including the three sub-groups differed markedly both in frequency and composition of alleles at different loci. On average, 77% of the loci were polymorphic with an overall mean of 2.2 alleles per locus and an observed heterozygosity of 0.387. The unbiased genetic identities (I) between groups ranged from 0.809 between Groups lb and 2 to 0.937 between Groups lb and lc. Group 2 has diverged from the other groups with an average genetic identity of 0.821. Summing over all 11 polymorphic loci, 16% of gene diversity was due to differentiation between groups and 84% to that within groups. Comparison of isozyme fingerprints revealed that some accessions identically named, such as ‘No mai tsz’, ‘Kwai mi’, and ‘Hak ip’, possessed different isozyme genotypes, whereas some others with different names displayed identical isozyme genotypes.

Technical Abstract: A genetic diversity analysis involving 49 lychee (Litchi chinensis SOM.) accessions using eight enzyme systems encoding 12 loci (Zdh-I, Zdh-2, Mdh-2, Per-l, Pgi-2, Pgm-1, Pgm-2, Sk& Tpi-1, Tpi-2, Ugpp-1, and Ugpp-2) revealed moderate to high levels of genetic variability. Cluster analysis of the isozyme data from 40 genetically different accessions of the total 49 identified three groups at the 50% level of genetic similarity, the largest of which contained 32 of the 40 accessions distributed in three sub-groups. The groups including the three sub-groups differed markedly both in frequency and composition of alleles at different loci. On average, 77% of the loci were polymorphic with an overall mean of 2.2 alleles per locus and an observed heterozygosity of 0.387. The unbiased genetic identities (I) between groups ranged from 0.809 between Groups lb and 2 to 0.937 between Groups lb and lc. Group 2 has diverged from the other groups with an average genetic identity of 0.821. Summing over all 11 polymorphic loci, 16% of gene diversity was due to differentiation between groups and 84% to that within groups. Comparison of isozyme fingerprints revealed that some accessions identically named, such as ‘No mai tsz’, ‘Kwai mi’, and ‘Hak ip’, possessed different isozyme genotypes, whereas some others with different names displayed identical isozyme genotypes.

Last Modified: 9/2/2014