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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF GENETIC RESOURCES FOR VITIS, PRUNUS, JUGLANS, FICUS, OLEA, PISTACIA, PUNICA, DIOSPYROS, ACTINIDIA, AND MORUS

Location: National Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes

Title: Enzyme polymorphisms in Canarium

Authors
item Sui, L. - UNIV OF HAWAII - HORT
item Zee, Francis
item Manshardt, R. - UNIV OF HAWAII - HORT
item Aradhya, Mallikarjuna

Submitted to: Scientia Horticulturae
Publication Type: Popular Publication
Publication Acceptance Date: September 17, 2006
Publication Date: August 1, 1997
Citation: Sui, L., Zee, F.T., Manshardt, R.M., Aradhya, M.K. 1997. Enzyme polymorphisms in Canarium. Scientia Horticultureae, 68: 197-206.

Interpretive Summary: Fifty-two accessions of Canarium involving seven species, C. ovatum, C. album, C. megalanthum, C. harveyi, C. indicum, C. mehenbethene, and C. odontophyllum were studied for isozyme polymorphisms. Starch gel electrophoresis with a histidine-citrate buffer system (pH 6.5) was employed to assay six enzymes: LAP, MDH, GPI, PGM, TPI and UGPP. Species were polymorphic for most of the six enzyme systems assayed and the level of phenotypic polymorphism for different species and enzymes assayed ranged from 0 to 0.88, with an overall average of 0.28. The weighted average of polymorphisms across different enzyme systems for different species ranged from 0.16 for C. indicum to 0.47 for C. ovatum, with an overall average of 0.26. On average, LAP was the most polymorphic enzyme (0.43) followed by GPI (0.38), MDH (0.27), UGPP (0.26), PGM (0.18), and TPI (0.13). The complexity of isozyme phenotypes indicates that interspecific hybridization and polyploidy might have played a major role in the evolution of Canarium species. Geographic speciation in Canarium was not apparent from the phonetic analysis of the isozyme data.

Technical Abstract: Fifty-two accessions of Canarium involving seven species, C. ovatum, C. album, C. megalanthum, C. harveyi, C. indicum, C. mehenbethene, and C. odontophyllum were studied for isozyme polymorphisms. Starch gel electrophoresis with a histidine-citrate buffer system (pH 6.5) was employed to assay six enzymes: LAP, MDH, GPI, PGM, TPI and UGPP. Species were polymorphic for most of the six enzyme systems assayed and the level of phenotypic polymorphism for different species and enzymes assayed ranged from 0 to 0.88, with an overall average of 0.28. The weighted average of polymorphisms across different enzyme systems for different species ranged from 0.16 for C. indicum to 0.47 for C. ovatum, with an overall average of 0.26. On average, LAP was the most polymorphic enzyme (0.43) followed by GPI (0.38), MDH (0.27), UGPP (0.26), PGM (0.18), and TPI (0.13). The complexity of isozyme phenotypes indicates that interspecific hybridization and polyploidy might have played a major role in the evolution of Canarium species. Geographic speciation in Canarium was not apparent from the phonetic analysis of the isozyme data.

Last Modified: 4/18/2014