ARS | Publication request: Putative regulatory elements within the non-coding regions of Chrysomelidae Diapause Associated Transcript-1 (DAT-1) orthologs

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2008
Publication Date: November 16, 2008
Citation: Toutges, M.J., Yocum, G.D., Roehrdanz, R.L. 2008. Putative regulatory elements within the non-coding regions of Chrysomelidae Diapause Associated Transcript-1 (DAT-1) orthologs. [Abstract]. Entomological Society of America Annual Meeting. Poster # D0177, p. 106.

Technical Abstract: To develop a more comprehensive understanding of diapause within Chrysomelidae, we are employing phylogenetic foot-printing to isolate and characterize the regulatory elements associated with the diapause-associated gene, DAT-1. Leptinotarsa decemlineata (Colorado potato beetle, CPB) DAT-1 has been previously elucidated and was used as the reference for L. juncta (false potato beetle, FPB). The nucleotide sequences from FPB and CPB were aligned, and analyzed. Both the FPB and the CPB contain highly conserved region 37 or 38 nucleotides (respectively) upstream of the TATA box. The nucleotide sequence CAACAAA found within the conserved region represents a response element that may associate with several transcription factors, suggesting a potential means for regulating DAT-1 gene expression. Additionally, the FPB and CPB DAT-1 genes reveal stringent conservation between these two species, with multiple putative regulatory elements found within the intronic regions of the genes.