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United States Department of Agriculture

Agricultural Research Service

Research Project: INSECT GENOMIC BIODIVERSITY AND MOLECULAR REGULATION OF DIAPAUSE

Location: Insect Genetics and Biochemistry Research

Title: Mitochondrial DNA divergence in Lygus lineolaris (Hemiptera: Miridae)

Authors
item Burange, Prasad - NORTH DAKOTA STATE UNIV.
item Roehrdanz, Richard
item Boetel, Mark - NORTH DAKOTA STATE UNIV.
item Sears Wichmann, Sheila

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2008
Publication Date: November 16, 2008
Citation: Burange, P.S., Roehrdanz, R.L., Boetel, M.A., Sears, S. 2008. Mitochondrial DNA Divergence in Lygus lineolaris (Hemiptera: Miridae) [abstract]. Entomological Society of America Annual Meeting. Poster No. D0540.

Technical Abstract: The genus Lygus is widely distributed in North America and Eurasia. The tarnished plant bug, Lygus lineolaris, is one of the most serious pest species within this genus having >300 different plant hosts. The intra-specific genetic diversity of L. lineolaris is being examined by employing mitochondrial (mt) and nuclear DNA markers. The cox1 barcode region and a larger mtDNA region overlapping cox1 and cox2 were sequenced. The complete L. lineolaris mt genome (17027 bp) has been determined. The arrangement of the mt genes matches the most common order for insects. The mt control region contains two sets of repeated units, six copies of 24 bp segment and five copies of a 161 bp sequence. The common mtDNA haplotypes are shared between widely dispersed populations, thus indicating a lack of strong geographically based population structure for this species. Neighbor-joining (NJ) trees suggest that most L. lineolaris individuals belong to two closely related clades showing a sympatric distribution. The prospect that these two clades might represent cryptic species is being investigated. The nuclear ribosomal ITS region is also being sequenced.

Last Modified: 10/1/2014
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