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United States Department of Agriculture

Agricultural Research Service

Title: Conversion of lower lignin mutants of Sorghum bicolor (L.) to ethanol

Authors
item Cotta, Michael
item Dien, Bruce

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 13, 2009
Publication Date: N/A

Technical Abstract: The United States Government has targeted aggressive development of ethanol as one route for decreasing oil dependence and lowering greenhouse gas emissions. Achieving future production targets depends on expanding feedstock sources beyond corn and towards lignocellulose. This is expected to include new low-input and high yielding energy crops. Utilizing dedicated energy crops allows for the possibility of breeding them for increased conversion efficiency to ethanol. In this study, the effect of reducing lignin content was evaluated for sugar and ethanol production in forage sorghum (Sorghum bicolor (L.)). The sample set included wild-type and brown midrib mutants for reduced lignin contents. The mutants had significantly reduced lignin contents compared to the wild-type, but did not significantly vary in carbohydrates. The set was treated with dilute-acid, washed, and the residual cellulose hydrolyzed with commercial cellulase. Extraction of glucose was negatively correlated with lignin content. Samples were again treated with dilute-acid, but this time the whole hydrolysate was neutralized and converted to ethanol using cellulases in combination with Saccharomyces yeast. The ethanol yield was also determined to be negatively correlated with lignin content. These results suggest that breeding for reduced lignin can lead to improved ethanol yields. In addition, selected samples were also evaluated using a more efficient alkaline pretreatment. Once again higher ethanol yields were associated with lower lignin contents.

Last Modified: 10/23/2014
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