|Kim, Duk Kyung - VIS SCI ANRI, ARS|
|Kim, Chul Hong - VIS SCI ANRI, ARS|
|Lamont, Susan - IOWA ST UNIV AMES,IA|
Submitted to: Avian Immunology Group Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 22, 2008
Publication Date: N/A
Technical Abstract: Two inbred chicken lines, M5.1 and M15.2 which are B-haplotype disparate Fayoumi chickens, show different disease phenotypes following oral infection with Eimeria maxima. Weight loss was reduced and fecal parasite numbers were lower in M5.1 compared with M15.2 line birds. This study was conducted to discriminate the gene expression profiles following Eimeria maxima infection between two B complex congenic lines of Fayoumi chickens which were appeared the differences in disease resistance and innate immunity against avian coccidiosis. cDNA microarray constructed from 9,668 EST of chicken intestinal intraepithelial lymphocytes (IEL) was used to compare the transcript levels in IELs of the M5.1 and M15.2 lines from uninfected and E. maxima infected birds. When compared to uninfected controls and using a cutoff of > 2.0-fold alteration, M5.1 demonstrated altered expression of 1 (down-regulated), 32 (6 up, 6 down), and 18 (5 up, 13 down) mRNAs at 3, 4, and 5 days post-infection respectively. In the case of the M15.2 line, altered expression was observed in 6 (3 up, 3 down), 29 (11 up, 18 down), and 32 (8 up, 24 down) transcripts at the 3 time points compared with uninfected controls. Comparison between infected M5.1 and M15.2 chickens revealed alterations in 32 (22 up, 10 down), 98 (55 up, 43 down), and 92 (59 up, 33 down) mRNAs at the 3 time points. Interestingly, of the genes with known chromosomal locations, the largest number of elements exhibiting > 2.0-fold altered expression levels between M5.1 and M15.2 were located on chromosome 1. In conclusion, transcriptional profiling data showed that more gene expression changes in M15.2 than M5.1, however, most differences between two chicken lines were exhibited at day 4 following E. maxima infection.