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United States Department of Agriculture

Agricultural Research Service

Research Project: Germplasm Enhancement and Genetic Improvement of Cotton

Location: Genetics and Precision Agriculture Research

Title: Constructing molecular linkage maps of chromosome 14sh and 22 sh and QTL mapping for major traits by use of substitution lines of Gossypium hirsutum L.

Authors
item Luan, Mingbao - COTTON RESEARCH INSTITUTE
item Guo, Xiangmo - COTTON RESEARCH INSTITUTE
item Zhang, Yongshan - COTTON RESEARCH INSTITUTE
item Yao, Jinbo - COTTON RESEARCH INSTITUTE
item Saha, Sukumar
item Jenkins, Johnie

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 2, 2008
Publication Date: July 10, 2008
Citation: Luan, M., Guo, X., Zhang, Y., Yao, J., Saha, S., Jenkins, J.N. 2008. Constructing molecular linkage map and chromosome 14sh and 33sh and QTL mapping for major traits by use of substitution lines of Gossypium hirsutum L. Cotton Science. 20(S):85.

Technical Abstract: It is an important approach in cotton improvement to transfer beneficial characters from sea island cotton (G.barbadense L.) to upland cotton(G. hirsutum L.) because of its high fiber quality. Recently a series of chromosome substitution lines(CS-B lines) were released in which a pair of Upland cotton chromosomes or chromosome arms were replaced by corresponding chromosomes or chromosome arms of sea island cotton. The creation of CS-B lines provided great contributions to cotton improvement and identification of beneficial genes. A high density molecular genetic map of the short arm of chromosome 22 was constructed from an F2 TM-1 X CSB22Sh population. A total of 4800 SSR primer pairs were used to screen polymorphism among the two parents, TM-1 and CSB22Sh, and their F1 progeny. The 51 polymorphic primer pairs were screened, and 65 polymorphic markers were amplified in the F2 population. Linkage test indicated that 63 markers are anchored in one linkage group, covering a total genetic distance of 89 cM. Thirty-three QTLs for important traits were mapped in the F2 and F2:3 generations in three environments. Thirteen of the 33 QTLs associated with agronomic and fiber traits were measured in two environments. Thirty-six epistasis QTLs related to agronomic and fiber traits were identified, among which 4 QTLs were stable. Eight condense regions were found. These results provide important information for utilizing important genes from the short arm of chromosome 22 in the MAS.

Last Modified: 7/30/2014
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