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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETICS, POPULATION BIOLOGY, AND HOST-PARASITE INTERACTIONS OF CEREAL RUST FUNGI AND THEIR DISEASES

Location: Cereal Disease Laboratory

Title: Virulence Evolution Within the Ug99 Lineage

Authors
item JIN, YUE
item SZABO, LES
item Pretorius, Z. - S. AFRICA, FREE STATE

Submitted to: Wheat Genetics International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 26, 2008
Publication Date: August 24, 2008
Citation: Jin, Y., Szabo, L.J., Pretorius, Z. 2008. Virulence Evolution Within the Ug99 Lineage. Wheat Genetics International Symposium Proceedings. Available: http://hdl.handle.net/2123/3435.

Interpretive Summary: Race Ug99 of the wheat stem rust pathogen, recognized for possessing virulence to Sr31, was first reported in Uganda in 1999. Based on the North American stem rust nomenclature system, Ug99 is identified as race TTKS. This race has been identified in Kenya, Ethiopia, Yemen, and Iran. We have identified several variants within this race group with virulence on Sr24, Sr36, and other avirulence/virulence combinations. We revised the North American stem rust nomenclature to enable it to recognize these virulence variations wthin this race group. DNA markers were used to analyze this group and results indicated that TTKS lineage in East Africa represent a distinct genetic lineage and that new variants may have arisen via mutation.

Technical Abstract: Race TTKSK (syn. Ug99) of Puccinia graminis f. sp. tritici, recognized for possessing virulence to the stem rust resistance gene Sr31, was first identified in Uganda in 1998. Since then, TTKSK has been identified in Kenya in 2005 and Yemen in 2006. In addition to virulence to Sr31, race TTKSK was found to be virulent to many resistant genes that are commonly used in breeding, and is considered as a major threat to wheat production in areas where stem rust occurs and a large number of current cultivars are susceptible. Two new races, TTKST with virulence to Sr24 and TTTSK with virulence to Sr36, were identified in Kenya in 2006 and 2007, respectively. The virulence profile of TTKST and TTTSK is similar to that of race TTKSK on most of the known stem rust resistance genes. Simple sequence repeat (SSR) markers were used to examine the genetic relationship between isolates of these races. All isolates of the "TTKS" group shared an identical SSR genotype. Virulence profile and SSR genotype suggest that the TTKS group is a distinct lineage and that races TTKST and TTTSK might be recent in origin. Because of their broad virulence to stem rust resistance genes in the adapted germplasm, these races present a major challenge for developing resistance in breeding.

Last Modified: 9/10/2014