ARS | Publication request: Expressed gene sequences from larval genes over-expressed upon Babesia bovis infection of Rhipecephalus (Boophilus) microplus

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: May 28, 2008
Publication Date: January 31, 2009
Repository URL: http://ncbi.nlm.nih.gov
Citation: Saldivar, L., Guerrero, F., Nene, V.M., Scoles, G.A. 2009. Expressed gene sequences from larval genes over-expressed upon Babesia bovis infection of Rhipecephalus (Boophilus) microplus. National Center for Biotechnology Information (NCBI). Available: http://ncbi.nlm.nih.gov. Assession numbers FG579553-FG579858.

Interpretive Summary: Rhipcephalus (Boophilus) microplus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We initiated a study of differential gene expression in Rhipicephalus (Boophilus) microplus following infection by Babesia bovis in an attempt to identify tick genes which might be critical to successful pathogen infection. These critical genes might be candidates for development of anti-Babesia control technologies. We utilized a technique called subtractive cDNA library synthesis which allowed the isolation of genes preferentially activated following the adult tick's infection with Babesia bovis. We allowed the tick's lifecycle to progress to the larval stage and isolated RNA from Babesia-infected larvae and uninfected larvae. These RNAs were utilized to produce subtracted cDNA libraries from which 306 expressed gene sequences were obtained. Thus, we have produced DNA sequence databases consisting of genes which are over-expressed in Babesia bovis-infected larvae compared to uninfected controls. The sets of sequences have been submitted and published under GenBank Accession Numbers FG579553-FG579858.

Technical Abstract: Boophilus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We utilized subtractive cDNA library synthesis techniques to obtain 306 DNA sequences from genes over-expressed in Babesis bovis-infected B. microplus larvae compared to uninfected control larvae. The sets of sequences have been submitted and published under GenBank Accession Numbers FG579553-FG579858.