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United States Department of Agriculture

Agricultural Research Service

Title: Using a surface plasmon resonance biosensor for rapid detection of salmonella typhimurium in chicken carcass

Authors
item Lan, Yubin
item Wang, Shizhou - TEXAS A&M UNIVERSITY
item Yin, Yongguang - JILIN UNIVERSITY
item Hoffmann, Wesley

Submitted to: Journal of Bionics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 15, 2008
Publication Date: September 1, 2008
Citation: Lan, Y., Wang, S., Yin, Y., Hoffmann, W.C. 2008. Using a surface plasmon resonance biosensor for rapid detection of Salmonella typhimurium in chicken carcass. Journal of Bionic Engineering. 5:239-246.

Interpretive Summary: Traditional testing methods for food borne pathogens in poultry, such as Salmonella, are relatively costly and time-consuming. It can take two to seven days to detect and confirm pathogenic bacteria in food, which is not acceptable for monitoring critical control points in processing facilities. A biosensor was used in this study to rapidly detect Salmonella on chicken carcasses. In order for the biosensor to work effectively, the methodology for preparing the biosensor was developed and described in this study. The biosensor will be an affordable tool for inspectors and quality control engineers to work in poultry industries and should decrease the time required to detect food borne pathogens during the processing cycle.

Technical Abstract: Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodborne pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody concentrations and dilution series of the organism. The SPR biosensor showed promising to detect existence of Salmonella Typhimurium at concentrations of 106 CFU/ml. Initial results show potential for its application for pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.

Last Modified: 4/19/2014