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Title: Microarray analysis of gene expression in acaricide-exposed Rhipcephalus (Boophilus) microplus larvae.

Author
item Saldivar, Leonel
item Guerrero, Felicito
item BRAYTON, KELLY - WASHINGTON STATE-PULLMAN

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 1/14/2008
Publication Date: 12/31/2008
Citation: Saldivar, L., Guerrero, F., Brayton, K.A. 2008. Microarray analysis of gene expression in acaricide-exposed Rhipcephalus (Boophilus) microplus larvae. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. GEO Submissions Series Record Number GSE10171, Sample Numbers GSM257121-257140.

Interpretive Summary: Rhipcephalus (Boophilus) microplus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We designed a set of experiments to examine genes of the southern cattle tick, Rhipicelphalus (Boophilus) microplus, emphasizing those genes which varied their level of expression in response to acaricide exposure. The acaricides used were coumaphos, permethrin, ivermectin, and amitraz, and these were applied in low doses to the tick's larval stage. Gene expression was quantified by using microarrays containing over 13,000 gene probes derived from a previously described R. microplus gene database acquired and analyzed at our laboratory. Among the differentially expressed genes were legumain, a gene involved in digestion of the tick's bloodmeal, glutathione S-transferase, a member of a family of enzymes which metabolize exogenous chemicals, and a gene with DNA sequence similarity to a tick salivary gland-associated protein. The microarray datasets have been submitted and published under GEO Submissions Series Record Number GSE10171, Sample Numbers GSM257121-257140.

Technical Abstract: Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13,000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus). Relative quantitative reverse transcriptase-PCR and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein. The microarray datasets have been submitted and published under GEO Submissions Series Record Number GSE10171, Sample Numbers GSM257121-257140.