CATFISH GENETICS, BREEDING, AND PHYSIOLOGY
Location: Catfish Genetics Research
Title: NRAMP, TNF, TLR5, and Hepcidin Expression in Resistant and Susceptible Families of Channel Catfish Following Challenge With E. ictaluri
Submitted to: Proceedings of International Congress on Biology of Fishes
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 2008
Publication Date: July 28, 2008
Citation: Booth, N.J., Peterson, B.C. 2008. NRAMP, TNF, TLR5, and Hepcidin Expression in Resistant and Susceptible Families of Channel Catfish Following Challenge With E. ictaluri. 2008, 8th International Congress on the Biology of Fishes, Abstracts, p. 12; Portland, OR, USA.
Real-time PCR was used to measure gene expression of Nramp, TNF, TLR5, and Hepcidin, in spleen and liver tissue from two families of channel catfish, one resistant and one susceptible to ESC, following challenge with Edwardsiella ictaluri. There were no significant differences in relative copy numbers of the four genes in either of the families at any time point. There were no significant differences in copy number of TNF or Hepcidin in uninfected control fish. TLR5 (P<0.01) and Nramp (P<0.0001) expression was significantly different in uninfected fish only at 6 hours post exposure (PE). Fold induction was similar in both families for Nramp and TLR5; however, Hepcidin demonstrated strong induction in the susceptible family at 72 hours PE, while TNF was strongly induced at 48 hours PE in the resistant fish. These results give insight into innate immune responses and suggest potential candidates for genetic markers of disease resistance.