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United States Department of Agriculture

Agricultural Research Service

Title: Microarray analysis of female- and larval-specific gene expression in the horn fly, Haematobia irritans

Authors
item Guerrero, Felix
item Dowd, Scot
item Sun, Ying Fang
item Saldivar, Leonel
item Wiley, Graham - U OF OKLAHOMA, NORMAN OK
item Macmil, Simone - U OF OKLAHOMA, NORMAN OK
item Najar, Fares - U OF OKLAHOMA, NORMAN OK
item Roe, Bruce - U OF OKLAHOMA, NORMAN OK
item Foil, L - LSU, BATON ROUGE, LA

Submitted to: Journal of Medical Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 12, 2008
Publication Date: March 1, 2012
Citation: Guerrero, F., Dowd, S.E., Sun, Y., Saldivar, L., Wiley, G.B., Macmil, S.L., Najar, F., Roe, B.A., Foil, L.D. 2012. Microarray analysis of female- and larval-specific gene expression in the horn fly, Haematobia irritans. Journal of Medical Entomology. 46(2):257-270.

Interpretive Summary: Novel population control technologies are being evaluated for their utility against agricultural pests. One of these technologies involves the use of dominant female conditional-lethal genetic systems. A critical need in this type of system is a highly expressed female-specific gene promoter which can be used to express large amounts of a lethality-inducing gene product in the insect of interest. There are no horn fly gene promoters identified to date which can serve this purpose. We designed microarray experiments from a recently developed horn fly EST database and probed to identify female-specific and larval-specific differential gene expression. We found 431 and 419 genes which were over- and under-expressed in adult female flies, respectively, compared to adult male flies. Additionally, 419 and 871 transcripts were over- and under-expressed in first instar larvae compared to adult flies. Three transcripts were identified which were over-expressed in adult females flies compared to adult males and which also were over-expressed in the first instar larval lifestage compared to adult flies. These three transcripts are candidates to provide the gene promoter for use in the dominant female conditional-lethal genetic system being designed for the horn fly.

Technical Abstract: Dominant conditional lethal gene systems are being investigated as population control technologies against agricultural insect pests. One of the critical components of these systems is a highly expressed female-specific gene promoter which can be used to drive expression of a lethality-inducing gene. To identify candidate genes to supply this gene promoter, microarrays were designed from a recently developed horn fly EST database and probed to identify female-specific and larval-specific differential gene expression. Analysis of dyeswap experiments found 431 and 419 transcripts which were over- and under-expressed in adult female flies, respectively, compared to adult male flies. Additionally, 419 and 871 transcripts were over- and under-expressed in first instar larvae compared to adult flies. Three transcripts were identified which were over-expressed in adult females flies compared to adult males and which also were over-expressed in the first instar larval lifestage compared to adult flies.

Last Modified: 8/30/2014
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