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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND APPLICATION OF MOLECULAR PHYLOGENETICS OF FUNGI TO ENHANCE FOOD SAFETY AND FOOD SECURITY Title: Trichothecene chemotype composition of Fusarium graminearum and related species in Finland and Russia

Authors
item O`donnell, Kerry
item Ward, Todd

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 2, 2008
Publication Date: September 2, 2008
Citation: O Donnell, K., Ward, T.J. 2008. Trichothecene chemotype composition of Fusarium graminearum and related species in Finland and Russia. Meeting Abstract.

Technical Abstract: Fusarium graminearum and type B trichothecene producers can be divided into three chemotypes. Analysis of 290 single-spore isolates of F. graminearum and related Fusarium species revealed that all F. graminearum isolates from Finland (15) and western Russian (26) possessed the 3ADON chemotype, while >90% of the isolates from southern Russia (45) possessed the 15ADON chemotype. In other parts of Russia and northern China both chemotypes were present. The only F. graminearum isolate with the NIV chemotype was from Germany. All 27 F. culmorum isolates (Finland and Russia) possessed the 3ADON chemotype, whereas all six isolates of F. cerealis possessed the NIV chemotype. These results are in accordance with the results of other chemotype-specific primers and mycotoxin analyses of pure cultures. In Finland there were no differences in the F. graminearum chemotype composition between the years 1986-93 and 2001-2004, while in the Far East (90 isolates) the 3ADON chemotype frequency increased between the years 1998-2006. This apparent shift in trichothecene chemotype frequency is similar to recently observed shifts in Fusarium head blight pathogen composition within North America. Two Russian F. graminearum isolates produced a positive signal with a 3ADON and 15ADON multilocus genotyping assay probe from opposite ends of the trichothecene gene cluster, suggesting that it may reflect recombination between isolates with these two chemotypes. Twelve isolates from the Far East and Siberia produced unusually low positive signals for the F. graminearum probes. These isolates likely harbor previously unrecognized variation at the probe sites and will be sequenced.

Last Modified: 12/25/2014
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