Technical Abstract: Net type of net blotch (NTNB) on barley, caused by Pyrenophora teres f. teres Drechs. is prevalent in barley growing regions worldwide and is particularly damaging under cool, wet conditions. A population of 118 doubled haploid (DH) lines developed from a cross between barley cultivars ‘Rika’ and ‘Kombar’ was used to evaluate resistance to NTNB due to their differential reaction to various isolates of P. teres f. teres. Rika was resistant to P. teres f. teres isolate 15A and susceptible to isolate 6A. Conversely, Kombar was resistant to 6A, but susceptible to 15A. A progeny isolate of a 15A × 6A cross identified as 15A×6A#4, which was virulent on both parental lines, was also used to evaluate the Rika/Kombar (RK) population. The RK DH population was evaluated for disease reactions to the three isolates. Isolate 15A induced a resistant: susceptible ratio of 78:40 (R: S) whereas isolate 6A induced a resistant: susceptible ratio of 40:78. All but two lines had opposite disease reactions indicating two major resistance genes linked in repulsion. Progeny isolate15A×6A#4 showed a resistant: susceptible ratio of 1:117 with the one resistant line also being the single line that was resistant to both 15A and 6A. Deviation from expected segregation ratios in the DH population was due to segregation distortion. An RK F2 population segregated in a 1:3 (R: S) ratio for both 15A and 6A indicating that resistance is recessive. Simple sequence repeat (SSR), sequence tagged site (STS), cleavage amplified polymorphism (CAP), and restriction fragment length polymorphism (RFLP) markers were used to identify a region on chromosome 6H associated with NTNB resistance in the RK DH population. Key words: NTNB, segregation distortion, and Drechslera teres.