Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 19, 2008
Publication Date: July 19, 2008
Citation: Fadly, A.M., Mays, J.K., Silva, R.F. 2008. Molecular analysis of reticuloendotheliosis virus isolates obtained from chickens, turkeys and prairie chickens located in different regions in the United States [abstract]. American Veterinary Medical Association Convention Notes. 145th AVMA Annual Convention, July 19-22, 2008, New Orleans, LA. 2008 CD-ROM. Technical Abstract: We have previously reported on the isolation of reticuloendotheliosis virus (REV) from broiler breeder chickens, turkeys and prairie chickens located in Alabama, California and Texas, respectively. In order to determine the genetic relationship among these REV isolates, one isolate from each species was chosen for molecular characterization. PCR primer sets were designed to amplify a 2135 base pair fragment overlapping the REV envelope region and partial long terminal repeat region. A high degree of DNA sequence homology between and among the three REV isolates was noted, demonstrating greater than 99.0% homology at the nucleotide level between the three isolates and strain CSV, a prototype of REV subtype C. Also, the percent homology for the amino acid sequence coding for the envelope protein between the three isolates and strain CSV of REV ranged from 97.8% to 99%. Results suggest that these REV isolates although obtained from different avian species located at three different locations in the USA are very closely related genetically.