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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #224943

Title: Molecular Variability of Grapevine leafroll associated virus-1 in the Pacific Northwest Vineyards

Author
item KARTHIKEYAN, G - WASHINGTON STATE UNIV
item MEKURIA, T - WASHINGTON STATE UNIV
item Martin, Robert
item NAIDU, R - WASHINGTON STATE UNIV

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2008
Publication Date: 6/1/2008
Citation: Karthikeyan, G., Mekuria, T., Martin, R.R., Naidu, R.A. 2008. Molecular variability of Grapevine leafroll associated virus-1 in the Pacific Northwest vineyards. Phytopathology. 98: S57.

Interpretive Summary:

Technical Abstract: Grapevine leafroll disease (GLD) is the most economically important viral disease in the Pacific Northwest. Grapevine leafroll-associated virus-1 (GLRaV-1) is one of six GLRaVs documented so far in the region. A study was undertaken to determine molecular variability of GLRaV-1 collected from two wine grape and two ornamental grape cultivars. A 398 nucleotide (nt) fragment specific to the coat protein duplicate 2 (CPd2) was amplified from eight GLRaV-1 isolates by reverse transcription-polymerase chain reaction (RT-PCR). In addition, a 633 nt fragment of the open reading frame 9 (ORF 9), located towards the 3’ end of the virus genome, was amplified by RT-PCR from the two ornamental grape cultivars. The CPd2- and ORF 9-specific amplicons were cloned and sequenced, and the sequences compared among themselves and with corresponding sequence of GLRaV-1 isolate from Australia (GenBank accession number AF195822). The CPd2 sequences showed nucleotide sequence identity between 93 and 99% and amino acid sequence identity between 91 and 100% among the eight GLRaV-1 isolates. However, these isolates showed 85-88% nucleotide sequence identity and 80-84% amino acid sequence identity with the corresponding CPd2 sequence of an Australian isolate. ORF 9 sequences from the two ornamental grapes showed 98% identity among themselves at both nucleotide and amino acid level and 88% identity with the Australian isolate at both nucleotide and amino acid level. These results suggest genetic diversity among GLRaV-1 isolates from the Pacific Northwest region and indicate their differences with GLRaV-1 isolate from Australia. Additional data on sequence variation in other genomic regions of GLRaV-1 will be presented.