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United States Department of Agriculture

Agricultural Research Service

Research Project: USING FUNCTIONAL AND APPLIED GENOMICS TO IMPROVE STRESS AND DISEASE RESISTANCE IN FRUIT TREES

Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection

Title: Identification of gene-specific markers for resistance to Erwinia amylovora (fire blight) in Malus (apple) by a functional genomics approach

Authors
item NORELLI, JOHN (JAY)
item Gardiner, Susan - HORT RESEARCH, NZ
item Malnoy, Mickael - CORNELL UNIV, GENEVA, NY
item Aldwinckle, Herb - CORNELL UNIV, GENEVA, NY
item Farrell, Jr, Robert - PENN STATE UNIV, YORK, PA
item Horner, Mary - HORT RESEARCH, NZ
item Celton, Jean-Mare - HORT RESEARCH, NZ
item BALDO, ANGELA
item Bowatta, Deepa - HORT RESEARCH, NZ
item Carlisle, Charmaine - HORT RESEARCH, NZ
item Lalli, Donna
item BASSETT, CAROLE
item WISNIEWSKI, MICHAEL

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: April 2, 2008
Publication Date: July 26, 2008
Citation: Norelli, J.L., Gardiner, S.E., Malnoy, M., Aldwinckle, H.S., Farrell, Jr, R.E., Horner, M., Celton, J., Baldo, A.M., Bowatta, D.R., Carlisle, C.M., Lalli, D., Bassett, C.L., Wisniewski, M.E. 2008. Identification of gene-specific markers for resistance to Erwinia amylovora (fire blight) in Malus (apple) by a functional genomics approach. American Phytopathological Society. 98:5114.

Technical Abstract: Fire blight, caused by Erwinia amylovora (Ea), is a destructive disease of apple (Malus), pear (Pyrus) and other plants in the rose family (Rosaceae). 650 expressed sequence tags (ESTs) associated with fire blight were identified from Ea-challenged apple leaf tissue by suppression subtractive hybridization (SSH) and cDNA-AFLP analysis. ESTs were ranked for their potential impact on resistance based on bioinformatics and inferences drawn from model systems. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers derived from highly ranked fire blight-associated ESTs were mapped in a 'M.9' x 'Robusta 5' population in which a major QTL for fire blight resistance has been located on Linkage Group 03. Markers for heat shock protein 90 (Hsp81-2), a secretory class III peroxidase and a serine/threonine-protein kinase mapped to the LG03 fire blight resistance QTL and reduced its size from 12cM to 4cM. Markers for a "putative disease resistance protein" (NCBI AY347778) and Skp1 (SCF-type E3 ubiquitin ligase) mapped to positions corresponding to the location of two QTLs reported in other populations (Calenge et al. 2005, Khan et al. 2006). To date, of 28 candidate fire blight resistance gene markers that have been mapped, 6 have co-located to or near known fire blight resistance QTLs. This research will facilitate new methods of marker-assisted selection to efficiently breed superior apple cultivars with fire blight resistance.

Last Modified: 8/19/2014
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