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United States Department of Agriculture

Agricultural Research Service

Title: Identification of SSR markers linked to rust resistance in Andean common bean PI 260418

Authors
item Pastor Corrales, Marcial
item Arraes, P - EMBRAPA-CNPAF, BRAZIL
item Lewers, Kimberly
item Vianello Brondani, R - EMBRAPA-CNPAF, BRAZIL
item Cortopassi Buso, G - EMBRAPA-CNPAF, BRAZIL
item Ferreira, M - EMBRAPA-CNPAF, BRAZIL
item Santos Martins, W - FED UNIV OF SOIAS, BRAZIL

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Proceedings
Publication Acceptance Date: March 20, 2008
Publication Date: June 20, 2008
Citation: Pastor Corrales, M.A., Arraes, P.A., Lewers, K.S., Vianello Brondani, R., Cortopassi Buso, G., Ferreira, M.A., Santos Martins, W. 2008. Identification of SSR markers linked to rust resistance in Andean common bean PI 260418. Bean Improvement Cooperative Annual Report. 51:46-47.

Technical Abstract: Bean rust is a major disease of common bean that reduces yields and increase costs of dry and snap bean production in many parts of the world. Disease resistant cultivars offer the most effective, economical, and environmentally-friendly method of controlling bean rust. However, this strategy is complicated by the high virulence diversity of the rust pathogen Uromyces appendiculatus. Many races of this pathogen have been identified and reported in all bean production areas of the world. Conversely, several rust resistance genes have been identified and characterized in common bean. All of these genes so far identified and published are dominant. Some of these genes, such as Ur-3, Ur-5, Ur-7, and Ur-11, as well as unnamed genes Ur-Dorado 108, Ur-Ouro Negro, Ur-BAC 6, and Ur-Dorado 53, are from Mesoamerican bean genotypes, while other genes, such as Ur-4, Ur-6, Ur-9, Ur-12, and Ur-13; as well as unnamed genes Ur-US#3 (Ur-8), Ur-Resisto (Ur-10) are from bean genotypes of the Andean gene pool. Invariably, rust resistance genes from the Mesoamerican gene pool have a broader spectrum of resistance than genes from the Andean gene pool. For instance, Mesoamerican genes Ur-3, Ur-5, and Ur-11 are resistant to 44, 70, and 89 races respectively, while Ur-4 and Ur-6 are resistant to 30 and 22 races respectively, of 90 races of the rust pathogen maintained at the USDA-ARS Bean Project in Beltsville, MD (Stavely, 2000). PI 250418 (collected in Bolivia) is the first Andean common bean with resistance to all but one of the same 90 races of the same bean rust pathogen mentioned above. PI 260418 is susceptible only to Andean race 84 which was collected from an Andean bean in Colorado. The reaction of PI 260418 to the 90 races of the rust pathogen resembles the reaction of PI 181996 (Ur-11) which is also resistant to all but one race (Mesoamerican race 108) of the same 90 races mentioned above. We have studied the inheritance of rust resistance in PI 260418 and now we endeavor to find molecular markers linked to this resistance for use in marker-assisted selection. Microsatellite markers are PCR-based markers that have been developed for a wide variety of plant species including commercial crops. Microsatellites detect length polymorphisms at genetic loci that have simple sequence repeats (SSR). The objective of this study was to identify molecular markers linked to the rust resistance gene or genes present in PI 260418. These markers will be very useful in the introgression of this new rust resistance into dry and snap bean cultivars.

Last Modified: 4/19/2014