ARS | Publication request: Tolerance to Cucurbit yellow stunting disorder virus in cucumber is not correlated with a delay in virus movement

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: May 20, 2008
Publication Date: July 26, 2008
Citation: Eid, S.G., Atamian, H.S., Abou-Jawdah, Y., Havey, M.J. 2008. Tolerance to Cucurbit yellow stunting disorder virus in cucumber is not correlated with a delay in virus movement [abstract]. American Phytopathological Society. 98:S9

Technical Abstract: Cucurbit yellow stunting disorder virus (CYSDV) is an emerging crinivirus causing significant yield losses to cucurbit crops. Previous studies showed that of over 120 cucumber accessions evaluated for resistance against CYSDV, no immune or highly resistant accessions were detected, but a limited number of accessions were tolerant and showed delayed expression of symptoms, milder final symptoms, and lower percentages of infected plants compared to susceptible varieties. This study evaluated the relative sensitivity of CYSDV serological detection methods as compared to nucleic acid based methods and reports that in a susceptible germplasm, CYSDV was detected 5 days post-inoculation (DPI) by RT-PCR or by tissue print immunoassay (TPIA), and 8-9 DPI by dot blot immunoassay (DBIA) or ELISA. Time course studies at 3, 5, 8 and 14 DPI, using TPIA, revealed that tolerance to CYSDV in three tolerant cucumber germplasms was not correlated with restricted or delayed virus movement.