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United States Department of Agriculture

Agricultural Research Service

Research Project: GERMPLASM DEVELOPMENT FOR SOUTHERN PLAINS RANGELAND AND PASTURE LANDSCAPES

Location: Rangeland and Pasture Research

Title: The use of ISSR markers to identify Texas bluegrass interspecific hybrids

Author
item GOLDMAN, JASON

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 2, 2008
Publication Date: November 19, 2008
Repository URL: http://hdl.handle.net/10113/37281
Citation: Goldman, J.J. 2008. The use of ISSR markers to identify Texas bluegrass interspecific hybrids. Plant Breeding. 127:644-646

Interpretive Summary: Texas bluegrass is a cool-season native grass species that contains separate male and female plants. Hybrids with other bluegrass species can be created by controlled movement of pollen from other bluegrass species onto female Texas bluegrass plants. Texas bluegrass x Kentucky bluegrass (TK)hybrids have the potential to produce turf-type material with a wider geographic range of adaption than pure Kentucky bluegrass. Hybrids are generally identified visually after they are established, based on inheritance of morphological/physiological traits from both parents. DNA based markers have the potential to identify hybrids prior to establishment. In this study, ISSR markers were tested on Texas bluegrass, Kentucky bluegrass, other bluegrass species and putative hybrids. Nine of the 17 primers tested consistently produced robust fingerprints and nine 2-way primer combinations were also selected. DNA fingerprints were highly reproducible and the majority of the selected primers (16/18) amplified hybrid profiles using two putative TK full-sib hybrids. Combined with a rapid DNA extraction protocol, the ISSR technique enabled a fast and practical way to detect F1 interspecific hybrids early in the breeding program and could also be useful for other applications that require DNA based markers.

Technical Abstract: Seventeen ISSR primers were screened on Texas bluegrass (Poa arachnifera), Poa species (Kentucky (KY) P. pratensis, Canadian(CA) P. compressa, Argentine (ARG) P. ligularis, and cv. Sherman, P. Secunda), and putative Texas x Kentucky (TK) and other hybrids to determine if they could be used to produce robust and reproducible DNA fingerprints and identify interspecific hybrids. Nine of the 17 primers consistently produced robust fingerprints and nine 2-way primer combinations were also selected. DNA fingerprints were highly reproducible and the majority of the selected primers (16/18) amplified hybrid profiles using two putative TK full-sib hybrids. Combined with a rapid DNA extraction protocol, the ISSR technique enabled a fast and practical way to detect F1 interspecific hybrids early in the breeding program and could also be useful for other applications that require DNA based markers.

Last Modified: 8/27/2014
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