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United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTIONS AND METHODOLOGIES TO REDUCE HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN CHICKENS

Location: Poultry Microbiological Safety Research

Title: Effect of sponges and gas-charged incubators on recovery of Campylobacter from broiler rinses

Author
item LINE, JOHN

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 22, 2008
Publication Date: September 20, 2008
Citation: Line, J.E. 2008. Effect of sponges and gas-charged incubators on recovery of Campylobacter from broiler rinses. Poultry Science Association Meeting Abstract. Vol. 87(suppl. 1) p. 122-123.

Technical Abstract: Campylobacter spp. are microaerophilic and capnophilic microorganisms. Methods to increase exposure of Campylobacter to appropriate microaerobic conditions could theoretically improve recovery of stressed cells. The porous nature of a sponge greatly increases the sample surface area exposed to microaerobic conditions when soaked in enrichment broth. We compared recovery of Campylobacter from retail chicken carcass rinses (n=30) in bags with and without added sterile sponges. The semi-permeable bags containing the samples and an equal volume of 2X Bolton Enrichment Broth were flushed and filled with Campy-gas (5% O2, 10% CO2 and 85% N2 ) and were placed in incubators under similar microaerobic or ambient atmospheric conditions. Results indicate that adding sponges to the enrichment bags had no significant effect on Campylobacter recovery. Likewise, there was no statistically significant difference in the percentage of positive samples recovered from gas-charged bags placed in microaerobic or ambient incubators.

Last Modified: 9/10/2014
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