|Tyagi, Neerja - NORTH DAKOTA STATE UNIV.|
Submitted to: World Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: February 21, 2008
Publication Date: June 10, 2008
Citation: Tyagi, N., Dahleen, L.S. 2008. Expression of Ethylene Biosynthesis Genes in Barley Tissue Culture. World Congress on In Vitro Biology. 44:S77-2063. Technical Abstract: The plant hormone ethylene influences green plant regeneration rates from barley callus cultures. Our studies have focused on the effects of short treatments of an ethylene inhibitor or an ethylene precursor on green plant regeneration from two barley cultivars and the expression patterns of two ethylene biosynthesis gene families, ACC (1-aminocyclopropane-1-carboxylicacid) synthase (ACS) and ACC oxidase (ACO), during different tissue culture stages. Calli from two barley cultivars, Morex and Golden Promise, were exposed to ten one-week treatments of ACC or AVG (aminoethoxyvinylglycine) during maintenance and regeneration stages of our culture system and ethylene production was compared to untreated control samples. Results indicate that ACC and AVG have a differential effect on regeneration in the two cultivars. Both the tissue culture stage and the AVG and ACC treatments influenced the amount of ethylene produced from callus. The expression of ACO and ACS genes is being tested in callus collected from control samples during the same maintenance and regeneration stages. Preliminary results have identified at least one constitutive ACO gene and one putative differentially expressed gene.