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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #222894

Title: Comparison of Aroma-active Compounds in Raspberry Bushy Dwarf Virus-Resistant Transgenic 'Meeker' Red Raspberries Using Stir Bar Sorptive Extraction-Gas Chromatography-Mass Spectrometry

Author
item MALOWICKI, SARAH M - OREGON STATE UNIVERSITY
item QIAN, M - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/9/2008
Publication Date: 8/5/2008
Citation: Malowicki, S.M., Qian, M.C., Martin, R.R. 2008. Comparison of aroma-active compounds in Raspberry bushy dwarf virus-resistant transgenic 'Meeker' red raspberries using stir bar sorptive extraction-gas chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry. 56:6648-6655.

Interpretive Summary: Fruit quality of transgenic and wild-type 'Meeker' red raspberry grown on research plots in Oregon and Washington was compared. The transgenic plants had been engineered to be resistant to Raspberry bushy dwarf virus (RBDV), which causes significant reduction in yield and quality in raspberry and raspberry-blackberry hybrids. Fruit was harvested in the 2004 and 2005 growing seasons. Year-to-year and site-to-site variations were observed for soluble solids and acidity, with Oregon raspberries having slightly higher soluble solids and lower acidity than Washington raspberries. Thirty volatile organic compounds were extracted using stir bar sorptive extraction (SBSE) and quantified with gas chromatography-mass spectroscopy (GC-MS). Much larger variations in the compounds analyzed were observed between planting sites and harvest seasons than between transgenic and wild-type raspberries.

Technical Abstract: Raspberry bushy dwarf virus (RBDV) causes significant reduction in yield and quality in raspberry and raspberry-blackberry hybrid. Genetic modifications were made to 'Meeker' red raspberries to impart RBDV resistance. The RBDV-resistant transgenic and wild type 'Meeker' plants were grown in Oregon and Washington, and the fruits were harvested in 2004 and 2005 growing seasons. Year-to-year and site-to-site variations were observed for °brix and titratable acidity, with Oregon raspberries having slightly higher °brix and lower titratable acidity than Washington raspberries. Thirty volatile compounds were quantified using stir bar sorptive extraction (SBSE) paired with gas chromatography-mass spectroscopy (GC-MS). There were very few differences in volatile concentrations between the transgenic varieties and the wild type 'Meeker'. Much larger variations were observed between sites and harvest seasons. Raspberries grown in Oregon appeared to have higher concentrations of d-octalactone, d-decalactone, geraniol, and linalool. Chiral analysis of a-ionone, a-pinene, linalool, terpinen-4-ol, d-octalactone and d-decalactone demonstrated a much higher percentage of one isomer over the other, particularly a-ionone, a-pinene, d-octalactone and d-decalactone, with more than 90% of one isomer, while a racemic mixture was observed for linalool. The isomeric analysis revealed very little variation between varieties, locations or years.