Submitted to: International Society for Horticultural Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: March 13, 2008
Publication Date: N/A
Technical Abstract: ‘Braeburn’ browning is an internal storage disorder sometimes associated with elevated storage CO2 concentrations. Incidence of the disorder can be reduced using pre-storage diphenylamine (DPA) drenches indicating a relationship between reactive chemical species and disorder occurrence. ‘Braeburn’ apples were sampled at commercial harvest. Accordingly, apples were treated with 2000 µL'L-1 DPA and stored at 1oC in air or in 1.5 kPa O2 and 1, 2, or 3 kPa CO2 for 1 or 2 months. The metabolic profile of damaged and healthy cortex samples was evaluated after harvest and following storage using GC-MS analysis of trimethylsilyl (oxime) derivatives. Principal component analysis of the metabolic profile revealed metabolomic shifts resulting from differing tissue integrity, diphenylamine treatment, hypoxic storage, CO2 concentration, and storage duration. This demonstrates that this subset of the metabolome is representative of metabolic perturbations associated with browning.