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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #222520

Title: Complete Nucleotide Sequence of an Isolate of Coleus vein necrosis virus from Verbena

Author
item KRAUS, J - OREGON STATE UNIVERSITY
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item PUTNAM, M - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/27/2008
Publication Date: 2/18/2008
Citation: Kraus, J., Tzanetakis, I.E., Putnam, M.L., Martin, R.R. 2008. Complete nucleotide sequence of an isolate of Coleus vein necrosis virus from Verbena. Archives of Virology. 152:381-384.

Interpretive Summary: Vegetatively propagated crops are particularly prone to virus infection, especially to viruses that can be transmitted by vectors that do well in commercial production environments, or mechanically transmitted viruses that are easily transmitted during plant pruning. Verbena is a popular ornamental plant that is susceptible to numerous viruses. A plant of 'Taylor Town Red' verbena exhibiting mottling, necrosis and low vigor was tested for the presence of viruses by extracting double-stranded RNA which is indicative of infection with an RNA virus. The dsRNA was cloned and sequenced and it was determined to be nearly identical to a partially sequenced carlavirus from Coleus, Coleus vein necrosis virus. The entire sequence of the virus from verbena was obtained and it is a typical carlavirus most closely related to Carnation latent virus, the type member of the Carlavirus genus. These results provide further information and rapid diagnostic tests that can be used to improve the plant health status of verbena and other susceptible hosts including coleus.

Technical Abstract: A plant of 'Taylor Town Red' verbena exhibiting mottling, necrosis and low vigor was tested for the presence of viruses by extracting double-stranded RNA which is indicative of infection with an RNA virus. The dsRNA was cloned and sequenced and a novel carlavirus identified. The new virus was determined to be nearly identical to a partially sequenced carlavirus from Coleus, coleus vein necrosis virus (CVNV). The entire sequence of the virus from verbena was obtained and it is a typical carlavirus with the coat protein (ORF-5) most closely related to carnation latent virus , the type member of the carlavirus genus. The isolate from veberna (CVNV-V) was 96% identical to the region of CVNV from coleus that has been sequenced. ORF1 is most closely related to poplar mosaic and garlic latent viruses. ORFs 2, 3 and 4 form the Triple Gene Block and are most closely related to poplar mosaic virus and ORF6 most closely related to garlic common latent virus.. Vegetatively propagated crops are particularly prone to virus infection, especially to viruses that can be transmitted by vectors that do well in commercial production environments, or mechanically transmitted viruses that are easily transmitted during plant pruning. Verbena is a popular ornamental plant that is susceptible to numerous viruses. These results provide further information and rapid diagnostic tests that can be used to improve the plant health status of verbena and other susceptible hosts including coleus.