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United States Department of Agriculture

Agricultural Research Service

Title: Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3

Authors
item Chang, In-Ae - KYUNGPOOK NATL UNIV KOREA
item Bae, Jae-Han - KYUNGPOOK NATL UNIV KOREA
item Suh, Min-Jung - KYUNGPOOK NATL UNIV KOREA
item Kim, In-Hwan - KOREA UNIV SEOUL KOREA
item Hou, Ching
item Kim, Hak-Ryul - KYUNGPOOK NATL UNIV KOREA

Submitted to: Applied Microbiology and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 22, 2007
Publication Date: January 22, 2008
Citation: Chang, I., Bae, J., Suh, M., Kim, I., Hou, C.T., Kim, H. 2008. Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3. Applied Microbiology and Biotechnology. 78:581-586.

Interpretive Summary: Previously, we discovered a new microbial strain PR3 that produced 7,10-dihydrox-fatty acid from oleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, and coatings materials. Now we found that strain PR3 can also convert triolein to 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity. Here, we focused on development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25oC, respectively. Magnesium ion was essentially required for DOD production. Other important conditions including inoculum size, time for substrate addition, substrate concentration were also studied. This new hydroxyl fatty acid is a value-added product from soybean oil and has potential industrial application.

Technical Abstract: Hydroxy fatty acids (HFA), originally found in small amounts mainly from plant systems, are well known to have special properties such as higher viscosity and reactivity compared with other normal fatty acids. These special properties make HFAs have high industrial potentials in a wide range of applications including resins, waxes, nylons, plastics, lubricants, cosmetics, and antimicrobial agents. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 (NRRL B-18602) are well known to utilize various unsaturated fatty acids to produce mono-, di-, and trihydroxy fatty acids. Previously we reported that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang IA, Kim IH, Kang SC, Hou CT, Kim HR 2007, Appl Microbiol Biotechnol 74:301-306). In this study, we focused on development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25oC, respectively. The Magnesium ion was essentially required for DOD production. Other important conditions including inoculum size, time for substrate addition, substrate concentration were also studied.

Last Modified: 11/21/2014
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