ARS | Publication request: Construction of a BAC Library of the Leymus Cinereus X L. Triticoides Hybrid

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 15, 2007
Publication Date: October 5, 2007
Citation: Larson, S.R., Scheuring, C., Bushman, B.S., Mott, I.W., Dong, J.J., Zhang, Y., Zhang, X., Zhang, H.B., Wang, R. 2007. Construction of a BAC Library of the Leymus Cinereus X L. Triticoides Hybrid. ASA-CSSA-SSSA Annual Meeting Abstracts.

Technical Abstract: Leymus cinereus and L. triticoides are two wildrye grasses with many contrasting morphological and agronomic traits. The interspecific hybrid Leymus cinereus X L. triticoides and its progenies had been used to develop linkage maps of traits and molecular markers. To generate genomic DNA sequence information for these important wildrye species, we have developed a bacterial artificial chromosome (BAC) library with two restriction enzymes (BamHI and MboI) from the nuclear DNA of the interspecific hybrid. The BamHI library consists of 313,728 clones arrayed in 817 384-well microplates and has an average insert size of 155.0 kb. The MboI library contains 92,160 clones arrayed in 240 384-well microplates and has an average insert size of 135.4 kb. Therefore, the combined library consists of 405,888 clones arrayed in 1,057 384-well microplates and has an average insert size of 150.5 kb. Since the genome of the hybrid is estimated to be about 10,000 Mb in size, the combined library is equivalent to approximately 6.1 x haploid genomes. All the clones of the library have been printed on 5 sets of the nylon membranes and will be screened with several sets of overgo probes to identify BAC clones containing candidate genes for traits of agronomic importance.