Technical Abstract: The purpose of the present study was to determine the effect of body weight on blood lipid profile, insulin resistance, and inflammatory biomarkers in Mexican-American children. Children (13.3 +/- 0.1 year) were recruited from a local school and assigned to one of three groups as a volunteer sample: healthy weight (HW) (>=10th and <85th BMI percentile; n = 42), at risk of overweight (RO) (>=85th and <95th; n = 25), or overweight (OW) (>=95th; n = 42). Plasma concentrations of hsCRP, sCD14, sIL-6R, sTNF-(alpha)R1, sTNF-(alpha)R2, IL-6, and TNF-alpha were determined by ELISA. OW children had significantly greater plasma concentrations of hsCRP (P = 0.003), sCD14 (P = 0.013), sIL-6R (P = 0.010), sTNF-(alpha)R1 (P < 0.001), sTNF-(alpha)R2 (P = 0.005), insulin (P = 0.001), TC:HDL ratio (P < 0.001), and triglycerides (P < 0.001) than HW children. Also plasma concentrations of hsCRP, sIL-6R, and sTNF-(alpha)R1 were significantly greater in OW compared with RO children. Overweight Mexican-American children had a higher concentration of inflammatory biomarkers than healthy weight children. To our knowledge, this is the first study to report that sCD14 is elevated in overweight compared with healthy weight Mexican-American children.