Technical Abstract: The effectiveness of reactive oxygen species (ROS) generating AirOcare equipment on the reduction of airborne bacteria in a meat processing environment was determined. Serratia marcescens and lactic acid bacteria (Lactococcus lactis subsp. lactis and Lactobacillus plantarum) were used to artificially contaminate the air using a 6-jet Collison nebulizer. Air in the meat processing room was sampled immediately after aerosol generation and at various predetermined times at multiple locations using a Staplex 6 stage air sampler. Approximately 4 log10 reduction of aerial S. marcescens population was observed within 2 hours of treatment (P<0.05) compared to 1 log10 reduction in control treatment. The S. marcescens populations reduced further by ~4.5 log10 after 24 h exposure of ROS treatment. Approx. 3 log10 CFU/m3 reductions in lactic acid bacteria were observed following 2-h exposure to ROS. Further exposure with ROS reduced lactic acid bacteria in air; however, the difference in their survival after 24 h exposure was not significantly different from control treatment. S. marcescens bacteria were more sensitive to ROS treatment than the lactic acid bacteria. These findings reveal that reactive oxygen species treatment using AirOcare unit significantly reduces airborne S. marcescens and lactic acid bacteria in meat processing environment within 2-h.