ARS | Publication request: Development and Characterization of Deletion and Mapping Populations for Functional Genomics and Rice Breeding

Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 15, 2008
Publication Date: March 1, 2008
Citation: Jia, Y., Rutger, J.N., Moldenhauer, K.K., Gibbons, J. 2008. Development and characterization of deletion and mapping populations for functional genomics and rice breeding. In: Proceedings of the 32nd Rice Technical Working Group Meetings, February 18-21, 2008, San Diego, CA. 2008. CDROM.

Technical Abstract: Traditionally, plant breeders improve the rice crop utilizing visible mutants possessing agronomically important traits. Mutations which occur in a single gene but do not necessarily result in an altered and/or detectable phenotype, are useful in discovering responsive genes. With accurate genome sequence, a large mutant population based on a single genetic background should facilitate the functional determination of predicted genes in a genome and should be useful for screening traits that are influenced by environmental stimuli. A large mutant population using US adapted Oryza sativa cv. Katy was developed using a combination of chemicals, fast neutrons and gamma irradiations. To date, 15,000 M4 have been recovered from fast neutron treatments. An additional 25,000 M2 recovered from EMS and gamma irradiations are being advanced to M4 using single seed descent breeding method. Currently, we have screened the population with blast strains to identify lines that are more susceptible and/or more resistant for identification of useful mutants for genetics study and crop improvement. In addition, several recombinant inbred line populations with parents containing different resistance genes are being developed using single seed descent method. These materials will be deposited at Genetic Stocks-Oryzae collection (GSOR) (http://ars.usda.gov/Main/docs.htm?docid=8318) at the Dale Bumpers National Rice Research Center, Stuttgart, AR. Currently, 15,000 M4 of Katy and the RIL populations of the cross of Raminad strain #3 (highly resistant to blast) with an experimental line RU9101001 (highly susceptible to blast), the cross of Katy with RU91010001, and the cross of Lemont (susceptible to sheath blight) and Jasmine 85 (resistant to sheath blight) are available for distribution. Progress on additional mapping populations that capture resistance genes from different genetic backgrounds and other traits for crop improvement will be presented.