ARS | Publication request: Characterization of recent H5N1 avian influenza isolates from Vietnam and evaluation of vaccine efficacy of commercial vaccines

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2008
Publication Date: July 19, 2003
Citation: Pfeiffer, J., Pantin Jackwood, M.J., Suarez, D.L. 2003. Characterization of recent H5N1 avian influenza isolates from Vietnam and evaluation of vaccine efficacy of commercial vaccines [abstract]. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 19-23, 2008, New Orleans, Louisiana. p. 42.

Technical Abstract: All eight gene segments of nineteen recent avian influenza (AI) virus isolates from Vietnam were sequenced, then phylogenetically and molecularly characterized. Based on the hemaggluinin (HA) gene, they formed 2 distinct sublineages within Clade 2. Additionally, the HAs of representative viruses from each subgroup were cloned into expression plasmids and used as DNA vaccines in chickens. Serum produced was used for antigenic characterization with the cross- hemagglutination inhibition (HI) test. Finally, a challenge study was performed to evaluate the efficacy of currently used vaccines in protecting chickens and ducks against clinical disease and viral shedding.