Technical Abstract: The Asian longhorned beetle (ALB), ‘Anoplophora glabripennis’, is one of the most economically and ecological important non-native, invasive forest pests recently discovered in North America. Despite the substantial impact of this pest, limited effort has been expended in regards to defining the genetic and molecular make-up of this species. Considering the significant role played by late-stadia larvae in host tree decimation, we undertook a small-scale EST sequencing project using a cDNA library constructed from 5th-instar ALB. The resultant dataset consisted of 599 “high quality” ESTs which, upon assembly, yielded 381 potentially unique transcripts. Each of these transcripts was catalogued as to putative molecular function, biological process, and associated cellular component according to the Gene Ontology classification system. Using this annotated dataset, we further highlighted a subset of assembled sequences associated with ‘A. glabripennis’ development and metamorphosis. This work advances the understanding of the diverse genomic, and molecular mechanisms which underlie coleopteran morphogenesis, which could lead to the development of novel control strategies for this important forest insect pest.