Technical Abstract: Ten Fragaria L. (strawberry) differentials for race determination of isolates of Phytophthora fragariae C.J. Hickman var. fragariae, the causal organism of red stele root rot disease, were molecularly characterized with previously published polymerase chain reaction (PCR) based sequence-characterized amplified polymorphism (SCAR) and simple sequence repeat (SSR) markers. The ten strawberry differentials were acquired from the National Clonal Germplasm Repository in Corvallis, OR, along with duplicates of two of the differentials, ‘Blakemore’ and Yaquina B, which were acquired indirectly from another collection at Plant Research International, Wageningen, Gelderland, Netherlands, for comparison and identity confirmation. These two sets of duplicates could not be distinguished molecularly, strongly suggesting they are indeed descended from the same clones. Two of the differentials, Yaquina A and Yaquina B, also could not be distinguished from each other molecularly, supporting recent reports that their reactions to the different pathogen races are similar and that there is very little, if any difference between these two accessions collected from Yaquina Bay, on the Oregon coast.