Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Identification of peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis

Authors
item Gomez, Michael - TEXAS TECH UNIVERSITY
item Burow, Gloria
item Burke, John
item Burow, Mark - TEXAS A&M EXPERIMENT STAT
item Denwar, Nicholas - TEXAS TECH UNIVERSITY
item Simpson, Charles - TAMU
item Ramasubramanian, T - CENTRAL RES INST FOR JUTE
item Puppala, N - NMSU

Submitted to: Peanut Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 13, 2008
Publication Date: October 1, 2008
Citation: Gomez, M., Burow, G.B., Burke, J.J., Burow, M., Denwar, N., Simpson, C., Ramasubramanian, T., Puppala, N. 2008. Identification of peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis. Peanut Science. 35(2):123-129.

Interpretive Summary: In peanut a self pollinating crop, hybridization is quite time consuming and challenging. A major problem is the identification of selfs form hybrids after crossing. To aid in distinguish selfs from hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparison of banding patterns to parents, 82% of the putative hybrids were demonstrated to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, resulting in a saving of resources otherwise used on propagation of selfs.

Technical Abstract: In peanut hybridization, it is very important to be able to distinguish selfs from true hybrids to save time and resources. To help facilitate this effort and provide molecular distinction between selfs from hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparison of banding patterns to parents, 82% of the putative hybrids were demonstrated to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, resulting in a saving of resources otherwise used on propagation of selfs.

Last Modified: 12/21/2014