Technical Abstract: Seventeen strains of the foodborne pathogen Listeria monocytogenes were tested for their ability to colonize alfalfa, radish, and broccoli sprouts, as well as their capacity to withstand acid and oxidative stress, two stresses common to the sprout growth environment. Whereas large variations in different strains’ abilities to colonize alfalfa sprouts were confirmed, the variations with radish and broccoli sprouts were less severe. With a few exceptions, strains that were poor colonizers of alfalfa tended to be among the low colonizers of radish and broccoli and vice versa. The strains showed variability in their resistances to both acid and oxidative stress. Statistical analysis showed that there was no correlation between acid stress and sprout colonization, but there was a positive correlation between resistance to oxidative stress and colonization of all three sprout types. While the response to oxidative stress is known to be important for L. monocytogenes virulence, it may also be important for life outside of a host.