|Sakakibara, Yoshikiyo - TSUKUBA, JAPAN|
Submitted to: UJNR Food & Agricultural Panel Proceedings
Publication Type: Proceedings
Publication Acceptance Date: October 21, 2007
Publication Date: October 21, 2007
Citation: Sakakibara, Y., Saha, B.C. 2007. Microbial conversion of L-arabinose to xylitol by coexpression of L-arabinose isomerase, D-tagatose 3-epimerase, and L-xylulose reductase in Escherichia coli. In: Proceedings of the U.S.-Japan Food and Agricultural Panel 36th Annual Meeting, October 21-25, 2007, Tsukuba, Ibaraki, Japan. p. 301-304. Technical Abstract: A microbial strain has been developed that can produce xylitol from L-arabinose at a high yield by transforming Escherichia coli with a new xylitol biosynthetic pathway consisting of L-arabinose isomerase, D-tagatose 3-epimerase, and L-xylulose reductase. An E. coli strain that heterologously expressed these enzymes produced 3.1 g/L xylitol with the yield of 0.66 g xylitol/g consumed L-arabinose. Moreover, utilization of glycerol as a co-substrate significantly improved the xylitol production and yield. The E. coli strain produced 10 g/L xylitol using 10 g/L L-arabinose and 15 g/L glycerol with the yield of 1.1 g xylitol/g consumed L-arabinose.