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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR TOOLS TO MINIMIZE RISK IN GENETICALLY ENGINEERED CROPS

Location: Crop Improvement & Utilization Research

Title: A Simple and Inexpensive Method for Sending Binary Vector Plasmid DNA by Mail

Authors
item Thilmony, Roger
item Thomson, James

Submitted to: Plant Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 28, 2008
Publication Date: July 1, 2008
Citation: Thilmony, R.L., Thomson, J.G. 2008. A simple and inexpensive method for sending binary vector plasmid dna by mail. Plant Biotechnology. 25:403-406.

Interpretive Summary: We describe a simple cost-effective technique for the transport of DNA by mail. Our results demonstrate that common multipurpose printing paper is a satisfactory substrate and superior to chromatography paper for the transport of DNA through the U.S. first class mail. We show that depositing as little as 100 ng of purified plasmid onto multipurpose printing paper is sufficient to allow recovery of the mailed DNA. This inexpensive method of DNA transport will be useful for global distribution of molecular biology materials between research labs.

Technical Abstract: We describe a simple cost-effective technique for the transport of plasmid DNA by mail. Our results demonstrate that common multipurpose printing paper is a satisfactory substrate and superior to the more absorbent 3MM chromatography paper for the transport of plasmid DNA through the U.S. first class mail. We show that depositing as little as 100 ng of purified plasmid onto multipurpose printing paper is sufficient to allow recovery of the mailed DNA. There was an inverse correlation between vector size and recovery of intact plasmid DNA.

Last Modified: 9/21/2014
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