Technical Abstract: A study was conducted to determine the effects of treating and reusing poultry chiller water in a commercial poultry processing facility. Broiler carcasses and chiller water were obtained from a commercial processing facility which had recently installed a TOMCO Pathogen Management SystemJ to recycle water in the middle section of a 3 compartment chiller. Carcasses were sampled prechill and post-chill (final exit) during the first and second shifts, while chiller water was sampled from the beginning and end of each of the 3 chiller compartments. Carcasses were subjected to a whole carcass rinse (WCR) in 0.1% peptone. Numbers of Escherichia coli (EC), coliforms (CF) and Campylobacter (CP) were determined from the WCR and chiller water samples. Prevalence of Salmonella (SAL) was also determined on the WCR and chiller water samples. On average, prechill levels of bacteria recovered from rinses were 2.6, 2.9 and 2.6 log10 cfu/mL for EC, CF and CP, respectively. Ten out of 40 (25%) prechill carcasses were positive for SAL. After chilling, numbers of EC, CF and CP recovered from carcass rinses decreased by 1.5, 1.5, and 2.0 log10 cfu/mL, respectively. However, 9 out of 40 (22%) post-chill carcasses were positive for SAL. When the chiller water samples were tested, counts of EC, CF and CP were found only in water collected from the first compartment of the chiller. Two out of 4 (50%) water samples collected from the entrance of the first compartment tested positive for SAL. The present study shows that poultry processing chiller water can be reused to cool poultry and still achieve reductions in numbers of bacteria (EC, CF and CP) or no increase in prevalence (SAL) of bacteria recovered from broiler carcasses.